Accutase - Gentle, Efficient, and Serum-Free Cell Dissociation in Biomedical Research

Accutase is a solution used in cell culture methods for dissociation of adherent cells from a culture surface. It’s a mix of proteolytic and collagenolytic enzymes, chiefly made of a protease known as Trypsin-like protease XIV and a protease thermolysin. Accutase is an instant solution that does not comprise any bacterial or mammalian derived components. Accutase is frequently chosen as a gentler substitute to trypsin, an alternative enzyme, which is commonly used for the process of cell dissociation.

These are some of the common advantages of making the use of Accutase and what makes this solution a smart and versatile choice as a solution for cell dissociation.

Gentle Cell Dissociation

Accutase is much gentler on cells and will not bring about much damage to the cells, as opposed to trypsin. Gentle cell dissociation is significant for more than a few reasons, chiefly when working with fragile and sensitive types of cells. With gentle dissociation approaches helps to sustain the health of the cell, function, and morphology, while diminishing the damage and cell stress.

Efficient Cell Dissociation

Apart from being gentle, Accutase also plays an effective role in detaching cells from the culture substrate. Accutase acts fairly rapidly to dissociate cells from culture surfaces, characteristically needing a smaller amount time than other enzymes for detachment of cells. This makes it a prevalent choice for routine cell passaging and other cell culture uses.

Effective Dissociation of Cells

Accutase can decrease cell clumping, improving cell survival and bring about more homogeneous cell populaces. And prominently, Accutase is known for conserving cell surface antigens, often lost throughout the usage of other dissociation approaches. This makes it a valued tool for experiments where the expression of surface markers is important, for example in flow cytometry or immunofluorescence examination.

There is No Requirement for Serum

Accutase can be put to use without serum, which is chiefly useful when we are working with serum-free media or when components of serum interfere with downstream assays. Furthermore, the enzymatic action of Accutase does not usually require to be quenched with serum. Different from trypsin, Accutase is tender enough that mere dilution with DPBS or cell culture media is needed to stop the dissociation action. (Where enzyme deactivation is essential, Soybean Trypsin Inhibitor is an efficient serum-free solution, which is well-suited with Accutase.)

Accutase Can be Used with Which Types of Cells?

Accutase is generally employed with a number of cell types, and is mainly useful for detaching delicate cell types, for example neuronal cells and pluripotent stem cells, along with for dissociating cells that are hard to separate from surfaces.

Reaching to a Conclusion

It is because of the rising focus on personalized medicine all over the globe, increasing support of the governments for cancer research, and also because of the increase in number of pharma and biotech companies, the demand for cell dissociation is on the rise. The total demand will reach a value of USD 1,347.0 million in the next seven years.

Top Key Players in the Cell Dissociation Industry Are:

• Thermo Fisher Scientific Inc.
• Becton, Dickinson and Company
• F. Hoffmann-La Roche Ltd
• STEMCELL Technologies Canada Inc.
• PromoCell GmbH
• VitaCyte LLC
• Gemini BioProducts LLC
• Innovative Cell Technologies Inc.
• Worthington Biochemical Corporation
• Capricorn Scientific

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